نانومقیاس

نانومقیاس

استخراج گزینش پذیر کروسین از زعفران با استفاده از نانوذرات مغناطیسی پلیمر قالب مولکولی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه شیمی تجزیه، دانشگئه شیمی، دانشگاه کاشان، کاشان، ایران
2 گروه آموزش شیمی، دانشگاه فرهنگیان، تهران، ایران
3 گروه شیمی تجزیه، دانشکده شیمی، دانشگاه کاشان، کاشان، ایران
چکیده
کروسین یکی از مواد مؤثره زعفران است که به‌طور مستقیم بر کیفیت و ارزش تجاری آن تأثیر می‌گذارد. در این پژوهش، یک روش ساده برای استخراج حساس و انتخابی کروسین از زعفران با استفاده از پلیمرهای قالب مولکولی مغناطیسی (MMIPs) توسعه داده شد. MMIPs با استفاده از جنتیوبیوز به‌عنوان مولکول الگو، متااکریلیک اسید به‌عنوان مونومر عاملی، اتیلن گلیکول دی‌متاکریلات به‌عنوان عامل شبکه ساز و Fe3O4@SiO2 به‌عنوان حامل مغناطیسی تهیه شد. مشخصه‌یابی نانوذرات با استفاده از طیف‌سنجی فروسرخ تبدیل فوریه (FT-IR)، پراش پرتو ایکس (XRD)، میکروسکوپ الکترونی روبشی (SEM)، مغناطیس‌سنج ارتعاشی (VSM) و آنالیز گرماوزنی (TGA) انجام شد. پارامترهای مختلف مؤثر بر راندمان استخراج، همدماهای جذب، سینتیک جذب و گزینش‌پذیری MMIPs مورد مطالعه قرار گرفت. آنالیت‌ها با استفاده از طیف‌سنجی مرئی-فرابنفش و کروماتوگرافی مایع با کارایی بالا تعیین شدند. نتایج نشان داد که مدل شبه مرتبه دوم جذب کروسین را توصیف می‌کند و داده‌های تعادلی جذب با مدل فروندلیچ مطابقت دارد. هم‌چنین جاذب ساختاری همگن داشت و جذب کروسین از طریق جذب برگشت‌پذیر چندلایه انجام شد. در شرایط بهینه، محدوده خطی mgL-1 0/50-05/0 بود. حد تشخیص و حد کمی‌سنجی به ترتیب mgL-1 014/0 و mgL-1 10/0 بود. روش پیشنهادی در استخراج کروسین از نمونه‌های حقیقی، نتایج رضایت‌بخشی را نشان داد.
کلیدواژه‌ها

موضوعات


عنوان مقاله English

Selective extraction of Crocin from Saffron using modified magnetic nanoparticles coated with molecularly imprinted polymer

نویسندگان English

Ali Gholami 1
Mahshid Golestaneh 2
Hedyeh Bigdeli Azari 3
1 Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran
2 Department of Chemistry Education, Farhangian University, Tehran, Iran
3 Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan, Iran
چکیده English

Crocin is one of effective ingredients of saffron, which directly affects saffron's quality and commercial value. Herein, a simple method has been developed based on magnetic molecularly imprinted polymers (MMIPs) for sensitive and selective extraction of crocin from saffron. MMIPs were prepared using gentiobiose as the template molecule, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linking agent, and Fe3O4@SiO2 as the magnetic support. Nanoparticle characterizations were performed using various techniques, including Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), vibrating sample magnetometry (VSM), and thermogravimetric analysis (TGA). Different parameters affecting extraction efficiency, isothermal absorption experiments, kinetics absorption experiments, and the selectivity of MMIPs was studied. The analytes were determined by UV-Vis spectrometry and high-performance liquid chromatography (HPLC). The result showed that the pseudo-second-order model describes the sorption of crocin, and the adsorption equilibrium data fit with the Freundlich model. Also, the sorbent had a homogeneous structure, and the crocin sorption occurred through multilayer reversible sorption. Under the optimized conditions, the linear range was between 0.05-50 mgL-1. The limit of detection and limit of quantification were 0.014 and 0.10 mgL-1, respectively. The proposed method in extracting crocin from real samples showed satisfactory results.

کلیدواژه‌ها English

Crocin
Saffron
Molecularly imprinted polymers
Magnetic nanoparticles
Selective extraction
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دوره 11، شماره 1
بهار 1403
صفحه 69-54

  • تاریخ دریافت 12 فروردین 1403
  • تاریخ پذیرش 09 اردیبهشت 1403