This study investigates the fabrication of an optical chip for use in a surface plasmon resonance (SPR) system, and evaluates its sensing performance for detection of various glucose concentrations. This type of sensor is based on the evanescent wave generated under total internal reflection conditions at the metal-dielectric interface. The chip consists of a glass substrate coated with a 50 nm gold thin film, along with a thin chromium adhesion layer to enhance the thin film-substrate bonding. The Au and Cr layers were deposited consecutively with no break using a thermal evaporation device (PVD) under high vacuum (5×10-5 mbar) with a uniform deposition rate of 0.1 nm/s. SEM and AFM analyses were employed to examine the thickness and surface topography of the chips. To determine the absorption characteristics, optical spectroscopy was carried out in the visible wavelength range. In the next step, reflection spectra and binding curves of distilled water to the surfaces of fabricated chips with different thicknesses were examined using Autolab ESPRIT device, and compared with a commercial chip. The optimal sample in terms of thickness, reflection spectrum and FWHM was found to be similar to the commercial chip, and selected for glucose detection experiments at concentrations of 5, 10, and 15 percent which the angular shifts for them were 1050, 1843, and 3035 millidegree, respectively. The repeatability of the sample was also examined and confirmed with an accuracy of one millidegree. The average sensitivity of the chip was 177 Deg/RIU. The results show that the optimal chip can be used as an equivalent to a commercially available product.