نانومقیاس

نانومقیاس

معرفی و بررسی زیست-فسفرین : از سنتز تا کاربرد در زیست‌پزشکی

نوع مقاله : مقاله پژوهشی

نویسندگان
1 دانشکده مهندسی برق وکامپیوتر؛ دانشگاه تهران؛ آزمایشگاه نانوالکترونیک
2 دانشکده مهندسی برق و کامپیوتر؛ دانشگاه تهران؛
چکیده
در این مقاله زیست-فسفرین، ترکیبی نوین بر پایه نانوصفحات فسفرین عاملدار شده با اسید سیالیک، به‌عنوان یک فسفرین پایدار و زیست‌سازگار برای کاربرد‌های زیست پزشکی معرفی شده‌است. سنتز و عاملدارسازی زیست-فسفرین بدون استفاده از حلال‌های شیمیایی سمی بررسی و مشخصه یابی‌های آن انجام گرفته‌است. همچنین قابلیت کاربردی زیست-فسفرین در درمان سرطان به‌عنوان نانوحامل دارویی و نیز عامل نور-گرما درمانی بررسی شده است. نتایج این تحقیق نشان می‌دهد: زیست-فسفرین در برابر اکسیداسیون پایدار بوده و دارای سمیت سلولی پایین است. علاوه بر این، نتایج آنالیزهای سلولی انجام شده جهت دارورسانی هدفمند و نور-گرما درمانی سلولهای سرطانی MDA-MB-231 با استفاده از دوکسوروبیسین لود شده بر‌‌‌روی “فسفرین زیستی” و تابش فروسرخ (IR) نشان می‌دهد که اگرچه نور-گرما درمانی با زیست-فسفرین به تنهایی قادر به کاهش درصد زنده‌مانی سلولهای سرطانی به ۷۶٪ می‌باشد اما اثرهم افزایی تابش IR بر دوکسوروبیسین لود شده بر روی زیست-فسفرین به عنوان حامل دارویی، بیشترین تأثیر را بر کاهش حیات سلولهای سرطانی داشته و میزان زنده‌مانی سلول‌ها را به 40٪ کاهش می‌دهد. این نتایج بیانگر قابلیت بالای زیست-فسفرین به‌عنوان یک نانوحامل دارویی مؤثر و قابل‌اعتماد در زیست‌پزشکی است.
کلیدواژه‌ها
موضوعات

عنوان مقاله English

Introduction and Exploration of Bio-phosphorene: From Synthesis to Biomedical Applications

نویسندگان English

Shamsoddin Mohajerzadeh 1
Nasrin Khazamipour 2
1 Nano-Electronic Lab, , School of Electrical and Computer Engineering, University of Tehran, Tehran, North kargar Ave, Campus No. 2,
2 Department of Electrical and Computer Engineering, University of Tehran, Iran
چکیده English

In this article, we introduce an innovative combination of phosphorene nanosheets functionalized by sialic acid, Apart from the functionalization of the phosphorene sheets with sialic acid, we have examined their biological effect on cancerous cells. The formation of crystalline phosphorene sheets is achieved using a hydrogen-assisted plasma processing. The functionalization of the phosphorene is feasible through immersion of the freshly prepared sheets in a sialic-acid containing solution, leading to the formation of covalent bonding of bio-molecule with the phosphorus atoms on the sheet. We have investigated the toxicity of the prepared sheets and their efficacy in drug delivery using a combination of material and biological assays. For the cancer cells, we have used MDA-MB-231 cell line which is a highly metastatic breast cancer line. We used Doxorubicin medication either loaded on bio-phosphorene or in a lonely administration.
Apart from high stability of the treated bio-phosphorene sheets, their toxicity and efficacy have been examined on the aforementioned cell line. We have used both MTT and flow cytometry analysis to observe the rate of survival or death of the treated cells using various methods. The addition of sialic-acid molecules to the bio-phosphorene leads to a constructive effect on the stability of the sheets in air and aqueous solution. The viability rate for the unloaded bio-phosphorene sheets on MDA-MB-231 cells remains as high as 90% if the concentration of bio-phosphorene sheets stands between 25 and 167 μg/mL. In addition, the viability drops to values close and smaller than 50%, if a combination of infra-red illumination and DOX drug treatment is used. By referring to the data obtained in this research, we believe the use of bio-phosphorene sheets can be considered as a vehicle for drug delivery to increase the death rate of highly cancerous cells, while less aggressive and healthy cells remain non-affected.

کلیدواژه‌ها English

Bio-phosphorene
sialic-acid
toxicity
viability
infrared
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دوره 12، شماره 2
تابستان 1404
صفحه 210-228

  • تاریخ دریافت 19 دی 1403
  • تاریخ بازنگری 15 تیر 1404
  • تاریخ پذیرش 26 شهریور 1404