In the present research, a rapid water-based preparation method is presented, which is a low-cost and environmentally friendly method for the large-scale production of MIL-53(Fe)-COOH. Then, by using the synthesized MOF, MIL-53(Fe)-COOH@PANI composite was prepared. To characterize these synthesized materials, scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and XRD were used. Finally, the adsorbent prepared was used to remove tetracycline from the aqueous solution. SEM images showed that these materials have very good porosity. The presence of MIL-53(Fe)-COOH substance was confirmed using XRD pattern and also the FTIR peaks were well matched with the prepared materials. In order to investigate the effect of the amount of absorbent, the experiments were conducted in amounts of 0.01 to 0.06 g/liter, and according to the results, 0.04 g/liter was selected as the optimal amount. In the series of experiments related to investigating the effect of tetracycline concentration, experiments were conducted with concentrations of 20 ppm to 60 ppm, and 40 ppm was selected as the optimal concentration. The best performance of the adsorbent material was observed at pH= 5 and the time was 40 minutes. In terms of reproducibility, the adsorbent showed relatively good performance.