In this paper, we study the electronic transport of an infinite ladder network that a part of it is twisted by using Green’s function method at the tight-binding approach. For this purpose, we insert the effects of orbital overlapping of the neighbor atoms as well as the effect of twisting, in the tight-binding parameters of the twisted part. Moreover, due to the symmetric considerations of the system Hamiltonian, the under consider problem reduces to the problem of the two simple chains. The numerical results show that the conductance dramatically depends on amount of twisting and the coefficient of variation of orbital overlapping strength. Increasing of twisting in the center ladder causes the decreasing of its energy window and consequently the resonance region becomes smaller.