نویسندگان
1 دانشکده علوم و فنون نوین، دانشگاه تهران، تهران
2 مرکز تحقیقات نانوبیوتکنولوژی- دانشگاه علوم پزشکی بقیةالله (ع)، تهران
3 مرکز تحقیقات نانوبیوتکنولوژی- دانشگاه علوم پزشکی بقیةالله (عج)، تهران
چکیده
کلیدواژه‌ها
عنوان مقاله [English]
نویسندگان [English]
Surface plasmon resonance SPR sensors are an optical platform capable of highly sensitive and specific measuring of biomolecular interactions in real-time. This label-free technology can quantify the kinetics, affinity and concentration of surface interactions. SPR sensors have been used to detect bacterial pathogens in clinical and food-related samples. SPR-based immunosensors that coupled with a specific antigen-antibody reaction, have become a promising tool for the quantification of bacteria as it offers sensitive, specific, rapid, and label-free detection. In this paper, we evaluated application of a SPR-based immunosensor for detection of Vibrio cholerae causative agent of cholera by a dual channel Surface plasmon resonance device. At first a Self Assembled MonolayerSAM of 11-Mercaptoundecanoic acid was formed on sensor chip and after that antibody against Vibrio cholera was immobilized on chip covalently. Then different dilutions of Vibrio cholera cultures was passed on immobilized antibody and the response of SPR was recorded. The specificity of this nanobiosensor was studied by interaction of some non Vibrio cholera bacteria.
کلیدواژه‌ها [English]
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