ساخت زیست حسگر الکتروشیمیایی شناسایی اتانول با استفاده از الکترود پلاتین اصلاح شده

نویسندگان

1 گروه بیوتکنولوژی، دانشکدهی مهندسی شیمی، دانشگاه صنعتی نوشیروانی بابل، بابل، مازندران

2 دانشکده ی مهندسی شیمی، دانشگاه آزاد اسلامی قوچان، قوچان، خراسان رضوی

چکیده

در این پژوهش، آنزیم الکل اکسیداز با استفاده از کیتوسان در سطح الکترود پلاتین تثبیت شد تا یک زیست حسگر الکتروشیمیایی دقیق و حساس برای شناسایی و اندازه گیری اتانول در فاز مایع طراحی شود. مطالعات آمپرومتری در محلول بافر فسفات M 1/0 حاوی اتانول انجام شد. باتوجه به نتایج به دست آمده، شرایط بهینه برای عملکرد زیست حسگر در دمای °C 35 و pH برابر با 8 حاصل شد. برای بهبود عملکرد زیست حسگر از نانوذرات طلا در ساخت آن استفاده شد و مقایسه ی نتایج حاصل از زیست حسگر قبل و بعد از افزودن این نانوذرات، افزایش چهار برابری در شدت جریان تولید شده از اکسایش اتانول را نشان داد. محدوده ی خطی زیست حسگر اصلاح شده با نانوذرات طلا، ثابت میکائیلیس- منتن و حساسیت آن نیز به ترتیب برابر با mM 0/016-2/66، mM 1 و µA/ mM cm2 2 محاسبه شدند.



عنوان مقاله [English]

Fabrication of Ethanol Detection Electrochemical Biosensor Using Modified Platinum Electrode

نویسندگان [English]

  • S. Khalegh Panah 1
  • M. Rahim Nejad 1
  • A. Farahbakhsh 2
  • R. Zokhtareh 1
چکیده [English]

In this research, alcohol oxidase was immobilized at platinum electrode surface using chitosan to design an accurate and sensitive electrochemical biosensor for detection and measurement of ethanol in liquid phase. Amperometric studies were carried out in 0.1 M phosphate buffer solution containing ethanol. According
to the obtained results, the optimal conditions for biosensor operation were acquired at the temperature of 35 °C and pH 8. In order to improve the biosensor performance, gold nanoparticles were used in its construction and the comparison of obtained results from designed biosensor before and after adding these nanoparticles showed four-fold enhancement in current intensity produced by ethanol oxidation. The linear range of the gold nanoparticles modified biosensor, its Michaelis-Menten constant value, and its sensitivity were calculated to be 0.016-2.66 mM, 1 mM, and 2 µA/ mM cm2 ,respectively.

کلیدواژه‌ها [English]

  • Electrochemical biosensor
  • Ethanol
  • Alcohol oxidase
  • Platinum electrode
  • Gold nanoparticles
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